Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation
Identifieur interne : 000838 ( Main/Exploration ); précédent : 000837; suivant : 000839Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation
Auteurs : Wonhwi Na [Corée du Sud] ; Dongwoo Nam [Corée du Sud] ; Hoyoon Lee [Corée du Sud] ; Sehyun Shin [Corée du Sud]Source :
- Biosensors & Bioelectronics [ 0956-5663 ] ; 2018.
Descripteurs français
- KwdFr :
- Agarose (), Amorces ADN (), Colorants fluorescents (), Facteurs temps, Humains, Hydrogels (), Limite de détection, Maladies virales (diagnostic), Microfluidique, Microsphères, Techniques d'amplification d'acides nucléiques, Techniques de biocapteur, Techniques de diagnostic moléculaire, Virus (isolement et purification).
- MESH :
- diagnostic : Maladies virales.
- isolement et purification : Virus.
- Agarose, Amorces ADN, Colorants fluorescents, Facteurs temps, Humains, Hydrogels, Limite de détection, Microfluidique, Microsphères, Techniques d'amplification d'acides nucléiques, Techniques de biocapteur, Techniques de diagnostic moléculaire.
English descriptors
- KwdEn :
- Biosensing Techniques, DNA Primers (chemistry), Fluorescent Dyes (chemistry), Humans, Hydrogels (chemistry), Limit of Detection, Microfluidics, Microspheres, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Sepharose (chemistry), Time Factors, Virus Diseases (diagnosis), Viruses (isolation & purification).
- MESH :
- chemical , chemistry : DNA Primers, Fluorescent Dyes, Hydrogels, Sepharose.
- diagnosis : Virus Diseases.
- isolation & purification : Viruses.
- Biosensing Techniques, Humans, Limit of Detection, Microfluidics, Microspheres, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Time Factors.
Abstract
There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.
Url:
DOI: 10.1016/j.bios.2018.02.040
PubMed: 29494886
PubMed Central: 7125521
Affiliations:
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Le document en format XML
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xml:lang="en" level="a" type="main">Rapid molecular diagnosis of infectious viruses in microfluidics using DNA hydrogel formation</title><author><name sortKey="Na, Wonhwi" sort="Na, Wonhwi" uniqKey="Na W" first="Wonhwi" last="Na">Wonhwi Na</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Micro/Nano Systems, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Micro/Nano Systems, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Nam, Dongwoo" sort="Nam, Dongwoo" uniqKey="Nam D" first="Dongwoo" last="Nam">Dongwoo Nam</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Mechanical Engineering, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Hoyoon" sort="Lee, Hoyoon" uniqKey="Lee H" first="Hoyoon" last="Lee">Hoyoon Lee</name><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Mechanical Engineering, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Shin, Sehyun" sort="Shin, Sehyun" uniqKey="Shin S" first="Sehyun" last="Shin">Sehyun Shin</name><affiliation wicri:level="1"><nlm:aff id="aff0005">Department of Micro/Nano Systems, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Micro/Nano Systems, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0010">Department of Mechanical Engineering, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Mechanical Engineering, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff0015">Nano-Biofluignostic Research Center, Korea University, Seoul 02841, Republic of Korea</nlm:aff><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Nano-Biofluignostic Research Center, Korea University, Seoul 02841</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Biosensors & Bioelectronics</title><idno type="ISSN">0956-5663</idno><idno type="eISSN">1873-4235</idno><imprint><date when="2018">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques</term><term>DNA Primers (chemistry)</term><term>Fluorescent Dyes (chemistry)</term><term>Humans</term><term>Hydrogels (chemistry)</term><term>Limit of Detection</term><term>Microfluidics</term><term>Microspheres</term><term>Molecular Diagnostic Techniques</term><term>Nucleic Acid Amplification Techniques</term><term>Sepharose (chemistry)</term><term>Time Factors</term><term>Virus Diseases (diagnosis)</term><term>Viruses (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agarose ()</term><term>Amorces ADN ()</term><term>Colorants fluorescents ()</term><term>Facteurs temps</term><term>Humains</term><term>Hydrogels ()</term><term>Limite de détection</term><term>Maladies virales (diagnostic)</term><term>Microfluidique</term><term>Microsphères</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de biocapteur</term><term>Techniques de diagnostic moléculaire</term><term>Virus (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA Primers</term><term>Fluorescent Dyes</term><term>Hydrogels</term><term>Sepharose</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Virus Diseases</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Maladies virales</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biosensing Techniques</term><term>Humans</term><term>Limit of Detection</term><term>Microfluidics</term><term>Microspheres</term><term>Molecular Diagnostic Techniques</term><term>Nucleic Acid Amplification Techniques</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Agarose</term><term>Amorces ADN</term><term>Colorants fluorescents</term><term>Facteurs temps</term><term>Humains</term><term>Hydrogels</term><term>Limite de détection</term><term>Microfluidique</term><term>Microsphères</term><term>Techniques d'amplification d'acides nucléiques</term><term>Techniques de biocapteur</term><term>Techniques de diagnostic moléculaire</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>There has been an urgent need to quickly screen and isolate patients with viral infections from patients with similar symptoms at point-of-care. In this study, we introduce a new microfluidic method for detection of various viruses using rolling circle amplification (RCA) of pathogens on the surface of thousands of microbeads packed in microchannels. When a targeted pathogen meets the corresponding particular template, the DNAs are rapidly amplified into a specific dumbbell shape through the RCA process, forming a DNA hydrogel and blocking the flow path formed between the beads. Due to the significant increase in reaction surface area, the detection time was shortened to less than 15 min and the detection limit of various pathogens has been reached to 0.1 pM. By injecting the stained liquid, the existence of the target pathogens in a sample fluid can be determined with the naked eye. Furthermore, by integrating multi-channel design, simultaneous phenotyping of various infective pathogens (i.e., Ebola, Middle East respiratory syndrome (MERS), and others) in biological specimens can be performed at a point-of-care.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Allegranzi, B" uniqKey="Allegranzi B">B. Allegranzi</name></author><author><name sortKey="Bagheri Nejad, S" uniqKey="Bagheri Nejad S">S. Bagheri Nejad</name></author><author><name sortKey="Combescure, C" uniqKey="Combescure C">C. Combescure</name></author><author><name sortKey="Graafmans, W" uniqKey="Graafmans W">W. Graafmans</name></author><author><name sortKey="Attar, H" uniqKey="Attar H">H. Attar</name></author><author><name sortKey="Donaldson, L" uniqKey="Donaldson L">L. Donaldson</name></author><author><name sortKey="Pittet, D" uniqKey="Pittet D">D. 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Ishii</name></author><author><name sortKey="Sasaki, N" uniqKey="Sasaki N">N. Sasaki</name></author><author><name sortKey="Sato, K" uniqKey="Sato K">K. Sato</name></author><author><name sortKey="Nilsson, M" uniqKey="Nilsson M">M. Nilsson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sato, K" uniqKey="Sato K">K. Sato</name></author><author><name sortKey="Tachihara, A" uniqKey="Tachihara A">A. Tachihara</name></author><author><name sortKey="Renberg, B" uniqKey="Renberg B">B. Renberg</name></author><author><name sortKey="Mawatari, K" uniqKey="Mawatari K">K. Mawatari</name></author><author><name sortKey="Sato, K" uniqKey="Sato K">K. Sato</name></author><author><name sortKey="Tanaka, Y" uniqKey="Tanaka Y">Y. Tanaka</name></author><author><name sortKey="Jarvius, J" uniqKey="Jarvius J">J. Jarvius</name></author><author><name sortKey="Nilsson, M" uniqKey="Nilsson M">M. Nilsson</name></author><author><name sortKey="Kitamori, T" uniqKey="Kitamori T">T. Kitamori</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Na, Wonhwi" sort="Na, Wonhwi" uniqKey="Na W" first="Wonhwi" last="Na">Wonhwi Na</name></region><name sortKey="Lee, Hoyoon" sort="Lee, Hoyoon" uniqKey="Lee H" first="Hoyoon" last="Lee">Hoyoon Lee</name><name sortKey="Nam, Dongwoo" sort="Nam, Dongwoo" uniqKey="Nam D" first="Dongwoo" last="Nam">Dongwoo Nam</name><name sortKey="Shin, Sehyun" sort="Shin, Sehyun" uniqKey="Shin S" first="Sehyun" last="Shin">Sehyun Shin</name><name sortKey="Shin, Sehyun" sort="Shin, Sehyun" uniqKey="Shin S" first="Sehyun" last="Shin">Sehyun Shin</name><name sortKey="Shin, Sehyun" sort="Shin, Sehyun" uniqKey="Shin S" first="Sehyun" last="Shin">Sehyun Shin</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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